主要配置1、暗箱主體：上下兩層，方便觀察和保存2、紫外可見透射箱：核酸凝膠面積20＊15cm3、紫外可見反射源：提供反射射紫外光4、對稱反射白光源：在下層暗箱頂部左右對稱設(shè)置5、白光墊板：拍攝蛋白凝膠等材料,樣品材料面積25＊20cm，可拍攝各種彩色材料6、可調(diào)式濾色裝置：可選擇590nm紫外濾色片或白光通透鎖定裝置 上層可鎖定,相機(jī)安全7、數(shù)碼相機(jī)凝膠拍攝說明書：nikon系列相機(jī)的拍攝說明書，可達(dá)到*拍攝效果8、相機(jī)轉(zhuǎn)節(jié)裝置：連接濾色裝置、固定相機(jī)9、紫外濾色片：進(jìn)口專業(yè)eb濾色片10、紫外觀察窗：防紫外，方便觀察選配件1、專業(yè)數(shù)碼相機(jī)：推薦nikon 4500 數(shù)碼相機(jī)，可拍攝黑白和彩色圖片。413萬象素選配件2、圖像采集卡：可在電腦同步觀察和拍攝選配件3、touching 2002 凝膠分析軟件：凝膠圖像的拍攝、管理、分出色的純化需要選擇一種以上的樹脂，bio–rad 將zui常用的一些樹脂以樣品檢測包形式提供給用戶，方便使用。 樣品包含有以下樹脂：• macro–prep high q,deae,high s 和cm, 各25ml • i 型和ii 型cht 陶瓷羥基磷灰石，各10 克 • unosphere q 和s, 各25ml • macro–prep 甲基hic 和t–丁基hic, 各25ml 目錄 # 描述158-0100 media sampler pack 用于自發(fā)固定配體的活化介質(zhì)使用戶能通過耦 聯(lián)一個抗體、酶、抗原或受體來制備所需要的親和 基質(zhì)?；罨橘|(zhì)靈活性，且使用方便。affi–gel 10 和affi–gel 15 介質(zhì)affi–gel 10 和affi–gel 15 親和介質(zhì)使配體與初 級胺發(fā)生自發(fā)、快速而有效的耦聯(lián)。affi–gel 10 載 體有效應(yīng)用于pi 6.5–11 的中性或堿性蛋白的耦聯(lián)。 affi–gel 15 凝膠用于耦聯(lián)pi<6.5 的酸性蛋白。affi–gel 10 和15 具有以下優(yōu)勢：• 水相或無機(jī)相的耦聯(lián)條件 • 4°c、4 小時內(nèi)完成蛋白耦聯(lián) • 蛋白耦聯(lián)量35 mg/ml凝膠 更多信息，請索取資料# 1085. affi–gel hz 活化載體affi–gel hz 活化載體通過結(jié)合抗體分子fc鏈的碳水 化合物部分完成對igg 分子的耦聯(lián)。這種結(jié)合產(chǎn)生 更特異的抗原–抗體作用，比其它介質(zhì)高出100– 300% 的抗原結(jié)合量。affi–gel hz載體特性包括：• 穩(wěn)定的共價腙腱 • 輕度氧化作用，不會改變抗體活性 • 抗原結(jié)合能力高 • ph穩(wěn)定性 蛋白耦聯(lián)于affi–gel 10 和affi–gel 15 凝膠。耦聯(lián)條件：每種蛋 白溶液（3 ml 0.1 m mops, ph 7.5, 含40 mg 蛋白）與2 ml affi– gel 凝膠結(jié)合。更多信息，請索取資料# 1424. 定向耦聯(lián)與隨機(jī)耦聯(lián)。免疫親和作用顯示定向耦聯(lián)affi–gel hz 凝膠與隨機(jī)耦聯(lián)cnbr 瓊脂糖載體 的效果比較。對所有親和載體，均建議在使用前做一個耦聯(lián)測試以檢測耦聯(lián)效率。目錄 # 描述affi-gel 10 and 15 media 153-1000 affi-gel 10 gel, 1 l 153-1500 affi-gel 15 gel, 1 l 153-6046 affi-gel 10 gel, 4 x 25 ml 153-6051 affi-gel 15 gel, 25 ml 153-6052 affi-gel 15 gel, 4 x 25 ml 153-6098 affi-gel 10/15 combination, includes 2 x 25 ml affi-gel 10 gel and 2 x 25 ml affi-gel 15 gel 153-6099 affi-gel 10 gel, 25 ml affi-gel hz activated support 153-6047 affi-gel hz hydrazide gel, 25 ml 153-6054 affi-gel hz 10x coupling buffer concentrate, 500 ml 153-6055 affi-gel oxidizer, 250 mg sodium periodate for use with affi-gel hz gel or affi-prep hz support 153-6060 affi-gel hz immunoaffinity kit, includes 5 ml affi-gel hz gel, 2 x 25 mg affi-gel hz oxidizer, 25 ml affi-gel hz coupling buffer concentrate, 2 econo-pac 10dg desalting columns, 1 x 10 cm econo-column column, instructions 即用型親和介質(zhì)的特異配體已與基質(zhì)耦聯(lián)，方便可靠。每個配體都有*的特異性來捕獲特定分子。 affi–gel 和affi–prep protein a 介質(zhì)affi–gel 和affi–prep protein a 層析介質(zhì)，可用于：獲得高純度的免疫球蛋白（igg），或其它免疫球蛋白分析前 選擇性地去除igg，或吸附免疫復(fù)合體以純化抗原。其中的protein a 可與免疫球蛋白，尤其哺乳類動物igg 的fc 鏈結(jié)合。功能優(yōu)勢包括：• 高純度的iggs • 與哺乳類動物igg 高親和性 • 使用maps 優(yōu)化緩沖液，對小鼠igg 、及其它亞類具有很高的載量 同時，affi–prep 介質(zhì)還體現(xiàn)了高線性流速zui大可達(dá)2,000 cm/hr，壓力穩(wěn)定zui大為1000 psi （ 70 bar ） 以及可用0.1 m naoh 清洗的高化學(xué)穩(wěn)定等性能。含有affi–gel、affi–prep protein a 介質(zhì)和maps ii 緩沖液的凝膠，每毫升可純化10 mg igg1，比標(biāo)準(zhǔn)方法高出8– 10 倍。另外，maps 處理中加入affi–gel protein a，與小鼠igg1 的結(jié)合比固定g 蛋白介質(zhì)要好。a 蛋白介質(zhì)載量________________________________________免疫球蛋白 affi–gel protein a 載量（mg/ml） affi–prep protein a 載量（mg/ml）小鼠igg1 6–10 7–9小鼠igg2a 6–10 7–9小鼠igg2b 6–10 7–9小鼠igg3 6–10 7–9小鼠igm＊ 6–10 7–9人igg 15 10–12綿羊igg 8–10 7–9牛igg 8–10 7–9馬igg 8–10 7–9山羊igg 8–10 7–9兔igg 8–10 7–9狗igg 8–10 7–9豬igg 8–10 7–9________________________________________＊ 使用maps 緩沖液系統(tǒng)，所有小鼠igms 的結(jié)合約為50%。affi–gel 藍(lán)膠affi–gel 藍(lán)膠是一種帶有共價結(jié)合cibacron blue f3ga 染料的交聯(lián)瓊脂糖 微球體。在很多應(yīng)用中，affi–gel 藍(lán)膠作為離子、疏水、芳香或空間活性的結(jié)合 位點(diǎn)，是去除白蛋白（50–100 目）及酶純化（100–200 目）的理想選擇。通過 調(diào)節(jié)洗脫液的組成，可對高特異性蛋白和多肽進(jìn)行結(jié)合和釋放。目錄 # 描述affi-gel and affi-prep protein a media 153-6153 affi-gel protein a support, 5 ml 153-6154 affi-gel protein a support, 50 ml 153-6159 affi-gel protein a maps ii kit, includes 5 ml affi-gel protein a agarose, affi-gel protein a maps ii buffers, 1 x 10 cm econo-column column; enough to purify 500 mg of mouse igg1 153-6161 protein a maps ii binding buffer, makes 5 l 156-0005 affi-prep protein a support, 25 ml 156-0006 affi-prep protein a support, 5 ml affi-gel blue gel 153-7301 affi-gel blue gel, 100 ml, 50–100 mesh （also available as prepacked econo-pac cartridges） 153-7302 affi-gel blue gel, 100 ml, 100–200 mesh deae affi-gel blue gel 153-7307 deae affi-gel blue gel, 100 ml cm affi-gel blue gel 153-7304 cm affi-gel blue gel, 100 ml affi-gel heparin gel 153-6173 affi-gel heparin gel, 40 ml affi-prep polymyxin support 156-0010 affi-prep polymyxin support, 25 ml affi-gel boronate gel 153-6103 affi-gel boronate gel, 5 g 153-6104 affi-gel boronate gel, 50 g cft ceramic fluoroapatite （ca10（po4）6（f2） is a composite of fluoroapatite and hydroxyapatite prepared by converting hydroxyapatite nanocrystals with a fluorine reagent. cft has separation characteristics similar to those of cht ceramic hydroxyapatite, and can be used under stringent chromatography conditions to separate acidic proteins requiring buffered conditions as low as ph 5, with minimal compromise of the solubility or lifetime of the support. cft is a rigid macroporous support and is available in two distinct material types, type i and type ii, and in one particle size, 40 µm. it has high binding capacity and may be used reproducibly over an extended number of cycles. its increased tensile strength, chemical durability, and density enable it to provide the throughput and consistent performance for all separations, including biopharmaceutical process-scale manufacturing. cft type i and type ii are chemically identical, but have been sintered at different times and temperatures, resulting in physically and chemically stable media. the dynamic capacity is higher for cft type ii than type i, the opposite of cht. protein se-lectivity is expected to be unaffected regardless of use of cft or cht. solubility or apatite lifetime, however, will be less of a concern with cft when lower ph buffered systems are a requirement. in order to determine which material types provide optimal chromatographic performance, evaluation of all four options — cft and cht, both types i and types ii — should be performed side by side.目錄 # 描述type i 157-0050 cft ceramic fluoroapatite, type i, 40 µm, 100 g 157-0051 cft ceramic fluoroapatite, type i, 40 µm, 1 kg 157-0055 cft ceramic fluoroapatite, type i, 40 µm, 5 kg type ii 157-5000 cft ceramic fluoroapatite, type ii, 40 µm, 100 g 157-5100 cft ceramic fluoroapatite, type ii, 40 µm, 1 kg 157-5500 cft ceramic fluoroapatite, type ii, 40 µm, 5 kg 158-5000 cft ceramic fluoroapatite, type i, 40 µm, 10 g 158-5200 cft ceramic fluoroapatite, type ii, 40 µm, 10 g bio–gel ht 凝膠— 是純化蛋白、核酸及其它大分子的理想產(chǎn)品。根據(jù)tiselius（1956）方法研制而成。bio–gel ht 凝膠懸浮于磷酸鈉緩沖液0.02% nan3 ，10 mm，4°c 保存。bio–gel htp 凝膠— bio–rad 根據(jù)tiselius 方法開發(fā)，經(jīng)過*處理程序干燥而成的材料?？墒覝乇４妗腋∮?緩沖液中的特性與bio–gel ht 凝膠類似；1g 可溶漲至2–3ml。dna 純bio–gel htp 凝膠— 一類顆粒大小比htp 凝膠小的干粉，極 大地增加了載量并提高對雙鏈dna 的選擇性。由于bio–gel htp 凝膠 的顆粒小，建議用分批式層析或短層析柱。目錄 # 描述130-0150 bio-gel ht hydroxyapatite, hydrated, 250 ml 130-0151 bio-gel ht hydroxyapatite, hydrated, 500 ml 130-0420 bio-gel htp hydroxyapatite, powder, 100 g 130-0421 bio-gel htp hydroxyapatite, powder, 1 kg 130-0425 bio-gel htp hydroxyapatite, powder, 5 kg 130-0520 bio-gel htp hydroxyapatite, dna grade, 100 g 737-6201 econo-column open-ended jacketed chromatography column, 1 x 30 cm, includes 2 flow adaptors for dna hydroxyapatite chromatography, 25 ml note: larger volumes and special packaging for industrial applications are available on request. 羥基磷灰石是化學(xué)和熱穩(wěn)定化合物，ph 耐性>5.5，可以廣泛地與 各種水相或有機(jī)相溶劑配合使用，并可用naoh 消毒和高壓滅菌。cht 陶瓷羥基磷灰石（原先稱為macro–prep 陶瓷羥基磷灰石）是一種球形、 巨孔的羥基磷灰石。它避免了晶體材料的局限性，可用于工業(yè)級層析柱。這種陶瓷材 料的分離方案與晶體羥基磷灰石的基本*。cht 既保留了晶體羥基磷灰石*的 分離特性，同時又可在高流速大型層析柱中反復(fù)使用，達(dá)數(shù)百循環(huán)。i 型和ii 型cht 陶瓷羥基磷灰石具有晶體羥基磷灰石類似的洗脫特性，但同時也 有3個重要差異。i型的蛋白載量高，且對酸性蛋白載量更高。ii型的蛋白載量較低， 對早期洗脫蛋白以及核酸的分辨率較佳。由于ii型對白蛋白的親和力很低，它通常比i 型更適于純化多種類的免疫球蛋白。3 種顆粒大小20, 40 和80μm 能廣泛地用于分析 規(guī)模到制備規(guī)模的應(yīng)用。目錄 # 描述cht ceramic hydroxyapatite, type i 157-0020 cht ceramic hydroxyapatite, type i, 20 µm particle size, 100 g 157-0021 cht ceramic hydroxyapatite, type i, 20 µm particle size, 1 kg 157-0025 cht ceramic hydroxyapatite, type i, 20 µm particle size, 5 kg 157-0040 cht ceramic hydroxyapatite, type i, 40 µm particle size, 100 g 157-0041 cht ceramic hydroxyapatite, type i, 40 µm particle size, 1 kg 157-0045 cht ceramic hydroxyapatite, type i, 40 µm particle size, 5 kg 157-0080 cht ceramic hydroxyapatite, type i, 80 µm particle size, 100 g 157-0081 cht ceramic hydroxyapatite, type i, 80 µm particle size, 1 kg 157-0085 cht ceramic hydroxyapatite, type i, 80 µm particle size, 5 kg 158-2000 cht ceramic hydroxyapatite, type i, 20 µm particle size, 10 g 158-4000 cht ceramic hydroxyapatite, type i, 40 µm particle size, 10 g 158-8000 cht ceramic hydroxyapatite, type i, 80 µm particle size, 10 g cht ceramic hydroxyapatite, type ii 157-2000 cht ceramic hydroxyapatite, type ii, 20 µm particle size, 100 g 157-2100 cht ceramic hydroxyapatite, type ii, 20 µm particle size, 1 kg 157-2500 cht ceramic hydroxyapatite, type ii, 20 µm particle size, 5 kg 157-4000 cht ceramic hydroxyapatite, type ii, 40 µm particle size, 100 g 157-4100 cht ceramic hydroxyapatite, type ii, 40 µm particle size, 1 kg 157-4500 cht ceramic hydroxyapatite, type ii, 40 µm particle size, 5 kg 157-8000 cht ceramic hydroxyapatite, type ii, 80 µm particle size, 100 g 157-8100 cht ceramic hydroxyapatite, type ii, 80 µm particle size, 1 kg 157-8500 cht ceramic hydroxyapatite, type ii, 80 µm particle size, 5 kg 158-2200 cht ceramic hydroxyapatite, type ii, 20 µm particle size, 10 g 158-4200 cht ceramic hydroxyapatite, type ii, 40 µm particle size, 10 g 158-8200 cht ceramic hydroxyapatite, type ii, 80 µm particle size, 10 g note: larger volumes and special packaging for industrial applications are available on request. bio–gel ht 凝膠— 是純化蛋白、核酸及其它大分子的理想產(chǎn)品。根據(jù)tiselius（1956）方法研制而成。bio–gel ht 凝膠懸浮于磷酸鈉緩沖液0.02% nan3 ，10 mm，4°c 保存。bio–gel htp 凝膠— bio–rad 根據(jù)tiselius 方法開發(fā)，經(jīng)過*處理程序干燥而成的材料?？墒覝乇４?。懸浮于 緩沖液中的特性與bio–gel ht 凝膠類似；1g 可溶漲至2–3ml。dna 純bio–gel htp 凝膠— 一類顆粒大小比htp 凝膠小的干粉，極 大地增加了載量并提高對雙鏈dna 的選擇性。由于bio–gel htp 凝膠 的顆粒小，建議用分批式層析或短層析柱。目錄 # 描述130-0150 bio-gel ht hydroxyapatite, hydrated, 250 ml 130-0151 bio-gel ht hydroxyapatite, hydrated, 500 ml 130-0420 bio-gel htp hydroxyapatite, powder, 100 g 130-0421 bio-gel htp hydroxyapatite, powder, 1 kg 130-0425 bio-gel htp hydroxyapatite, powder, 5 kg 130-0520 bio-gel htp hydroxyapatite, dna grade, 100 g 737-6201 econo-column open-ended jacketed chromatography column, 1 x 30 cm, includes 2 flow adaptors for dna hydroxyapatite chromatography, 25 ml note: larger volumes and special packaging for industrial applications are available on request. 羥基磷灰石是化學(xué)和熱穩(wěn)定化合物，ph 耐性>5.5，可以廣泛地與 各種水相或有機(jī)相溶劑配合使用，并可用naoh 消毒和高壓滅菌。cht 陶瓷羥基磷灰石（原先稱為macro–prep 陶瓷羥基磷灰石）是一種球形、 巨孔的羥基磷灰石。它避免了晶體材料的局限性，可用于工業(yè)級層析柱。這種陶瓷材 料的分離方案與晶體羥基磷灰石的基本*。cht 既保留了晶體羥基磷灰石*的 分離特性，同時又可在高流速大型層析柱中反復(fù)使用，達(dá)數(shù)百循環(huán)。i 型和ii 型cht 陶瓷羥基磷灰石具有晶體羥基磷灰石類似的洗脫特性，但同時也 有3個重要差異。i型的蛋白載量高，且對酸性蛋白載量更高。ii型的蛋白載量較低， 對早期洗脫蛋白以及核酸的分辨率較佳。由于ii型對白蛋白的親和力很低，它通常比i 型更適于純化多種類的免疫球蛋白。3 種顆粒大小20, 40 和80μm 能廣泛地用于分析 規(guī)模到制備規(guī)模的應(yīng)用。目錄 # 描述cht ceramic hydroxyapatite, type i 157-0020 cht ceramic hydroxyapatite, type i, 20 µm particle size, 100 g 157-0021 cht ceramic hydroxyapatite, type i, 20 µm particle size, 1 kg 157-0025 cht ceramic hydroxyapatite, type i, 20 µm particle size, 5 kg 157-0040 cht ceramic hydroxyapatite, type i, 40 µm particle size, 100 g 157-0041 cht ceramic hydroxyapatite, type i, 40 µm particle size, 1 kg 157-0045 cht ceramic hydroxyapatite, type i, 40 µm particle size, 5 kg 157-0080 cht ceramic hydroxyapatite, type i, 80 µm particle size, 100 g 157-0081 cht ceramic hydroxyapatite, type i, 80 µm particle size, 1 kg 157-0085 cht ceramic hydroxyapatite, type i, 80 µm particle size, 5 kg 158-2000 cht ceramic hydroxyapatite, type i, 20 µm particle size, 10 g 158-4000 cht ceramic hydroxyapatite, type i, 40 µm particle size, 10 g 158-8000 cht ceramic hydroxyapatite, type i, 80 µm particle size, 10 g cht ceramic hydroxyapatite, type ii 157-2000 cht ceramic hydroxyapatite, type ii, 20 µm particle size, 100 g 157-2100 cht ceramic hydroxyapatite, type ii, 20 µm particle size, 1 kg 157-2500 cht ceramic hydroxyapatite, type ii, 20 µm particle size, 5 kg 157-4000 cht ceramic hydroxyapatite, type ii, 40 µm particle size, 100 g 157-4100 cht ceramic hydroxyapatite, type ii, 40 µm particle size, 1 kg 157-4500 cht ceramic hydroxyapatite, type ii, 40 µm particle size, 5 kg 157-8000 cht ceramic hydroxyapatite, type ii, 80 µm particle size, 100 g 157-8100 cht ceramic hydroxyapatite, type ii, 80 µm particle size, 1 kg 157-8500 cht ceramic hydroxyapatite, type ii, 80 µm particle size, 5 kg 158-2200 cht ceramic hydroxyapatite, type ii, 20 µm particle size, 10 g 158-4200 cht ceramic hydroxyapatite, type ii, 40 µm particle size, 10 g 158-8200 cht ceramic hydroxyapatite, type ii, 80 µm particle size, 10 g note: larger volumes and special packaging for industrial applications are available on request. 分子生物純樹脂與分析純樹脂的化學(xué)性質(zhì)相當(dāng)，確保不含核酸內(nèi)切酶、外切酶以及連接酶抑制劑。 • ag 50w–x8 強(qiáng)陽離子交換樹脂— 具有*的去除dna 樣品中溴化乙錠和碘化丙錠的作用 • ag 501–x8 混合床樹脂— 用于水和非電解質(zhì)溶液的去離子化 • chelex 100 分子生物純樹脂— 顆粒度范圍200–400 目，適于重懸后的簡單轉(zhuǎn)移。50g包裝適于小規(guī)模試劑用戶，并附分析證書 • 生物工藝純樹脂— 經(jīng)特殊處理，每克微生物含量少于100 個 目錄 # 描述molecular biology grade media 142-1253 chelex 100 resin, molecular biology grade, 50 g, sodium, 200–400 dry mesh, 75–150 &181;m wet bead 143-1441 ag 50w-x8 resin, molecular biology grade, 100 g, sodium, 100–200 dry mesh, 106–250 µm wet bead 143-1451 ag 50w-x8 resin, molecular biology grade, 100 g, sodium, 200–400 dry mesh, 63–150 µm wet bead 143-6424 ag 501-x8 resin, molecular biology grade, 100 g, h+ + oh-, 20–50 dry mesh, 300–1,180 µm wet bead 143-6425 ag 501-x8（d） resin, molecular biology grade, 100 g, h+ + oh-, 20–50 dry mesh, 300–1,180 µm wet bead biotechnology grade media 143-1255 ag 1-x2 resin, biotechnology grade, 100 g, hydroxide, 200–400 dry mesh, 75–180 µm wet bead 143-1345 ag 1-x4 resin, biotechnology grade, 100 g, hydroxide, 100–200 dry mesh, 63–150 µm wet bead 143-2445 ag 1-x8 resin, biotechnology grade, 100 g, hydroxide, 100–200 dry mesh, 106–180 µm wet bead 143-2446 ag 1-x8 resin, biotechnology grade, 100 g, hydroxide, 200–400 dry mesh, 45–106 µm wet bead 143-2832 chelex 100 resin, biotechnology grade, 100 g, sodium, 100–200 dry mesh, 150–300 µm wet bead 143-3341 ag 4-x4 resin, biotechnology grade, 100 g, free base, 100–200 dry mesh, 75–150 µm wet bead 143-5241 ag 50w-x2 resin, biotechnology grade, 100 g, hydrogen, 100–200 dry mesh, 106–300 µm wet bead 143-5341 ag 50w-x4 resin, biotechnology grade, 100 g, hydroxide, 200–400 dry mesh, 75–150 µm wet bead 143-5441 ag 50w-x8 resin, biotechnology grade, 100 g, hydroxide, 100–200 dry mesh, 106–250 µm wet bead 143-5451 ag 50w-x8 resin, biotechnology grade, 100 g, hydroxide, 200–400 dry mesh, 63–150 µm wet bead 143-5832 bio-rex 70 resin, biotechnology grade, 100 g, sodium, 50–100 dry mesh, 150–300 µm wet bead 143-5852 bio-rex 70 resin, biotechnology grade, 100 g, sodium, 200–400 dry mesh, 45–75 µm wet bead 143-7424 ag 501-x8 resin, biotechnology grade, 100 g, h+ + oh-, 20–50 dry mesh, 300–1,180 µm wet bead 143-7425 ag 501-x8（d） resin, biotechnology grade, 100 g, h+ + oh-, 20–50 dry mesh, 300–1,180 µm wet bead 143-7834 ag 11 a8 resin, biotechnology grade, 100 g, self-adsorbed, 50–100 dry mesh, 180–425 µm wet bead ag 分析純樹脂— 是經(jīng)過嚴(yán)格純化，去除了有機(jī)和無機(jī)雜質(zhì)的層析介質(zhì)。樹脂按大小分類，確保濕膠顆粒度穩(wěn)定 地控制在一個狹小范圍內(nèi)，從而提供良好的分辨率和重復(fù)性。各種ag 樹脂均可以作為強(qiáng)（弱）陽離子（陰離子） 交換劑和混合基質(zhì)離子交換劑，許多介質(zhì)具有多種離子形式，并可從一種形式轉(zhuǎn)變成另一種。ag 樹脂主要用于分 離小分子化合物，包括無機(jī)離子、有機(jī)酸、核酸和醣類。bio–rex 樹脂— 作為弱陰離子和陽離子交換劑以及單一規(guī)格的混合基質(zhì)離子交換劑。bio–rex 70 樹脂是一種對 大分子有很好載量的大網(wǎng)狀結(jié)構(gòu)樹脂，用于純化、分離蛋白與多肽。chelex 樹脂— 含有成對的二乙酸亞胺離子與聚苯乙烯二乙烯基苯載體結(jié)合。它們是*的螯合樹脂，可以高選擇性 地結(jié)合多價陽離子，并可用于去除樣品和緩沖液中的金屬離子。分析純樹脂濕膠顆粒度和對應(yīng)的直徑________________________________________濕膠顆粒度（us 標(biāo)準(zhǔn)） 16 20 40 50 80 100 140 200 270 325 400直徑, μm 1,180 850 425 300 180 150 106 75 53 45 38________________________________________分析純樹脂選擇指南________________________________________交聯(lián)度 排阻分子量大小 操作穩(wěn)定性/ ph范圍 zui小濕載量（meq/ml） 標(biāo)準(zhǔn)密度 （g/ml） 離子形式ag 1 樹脂 類型：強(qiáng)陰離子 2% 2,700 0–14/0–14 0.6 0.65 氧化物，醋酸鹽官能團(tuán)：r–ch2n–（ch3）3 > 4% 1,400 1.0 0.7 氧化物基質(zhì)：聚苯乙烯二乙烯基苯 8% 1,000 1.2 0.75 氧化物，氫氧化物醋酸鹽，甲酸鹽應(yīng)用：交換鹽或兩性電解質(zhì)堿性形式（ph> pi）的陰離子。低交聯(lián)度用于高分子量物質(zhì)如多肽、核苷酸及鈾化合物。高交聯(lián)度用于低分子量無機(jī)陰離子以及環(huán)核苷酸測試和有機(jī)酸分離等的應(yīng)用。ag 2 樹脂 類型：強(qiáng)陰離子 8% 1,000 0–14/0–14 1.2 0.75 氧化物官能團(tuán)：r–ch2n+（ch3）2c2h4oh 基質(zhì)：聚苯乙烯二乙烯基苯 應(yīng)用：類似于ag 1 樹脂，但堿性和抗氧化性能較弱。ag 2 樹脂可分離糖、糖醇和配糖體。有些與ag 1 樹脂會發(fā)生異構(gòu)化的糖，不會與ag 2 樹脂發(fā)生。ag 50w 樹脂 類型：強(qiáng)陽離子 2% 2,700 0–14/0–14 0.6 0.7 氫官能團(tuán)：r–so3– 4% 1,400 1.1 0.8 氫基質(zhì)：聚苯乙烯二乙烯基苯 8% 12% 1,000400 1.72.1 0.80.85 氫氫應(yīng)用：低交聯(lián)度樹脂用于分離或濃縮多肽、核苷酸和氨基酸。高交聯(lián)度樹脂用于分離小肽和氨基酸，去除陽離子和分離金屬離子。ag mp–1m 樹脂 類型：強(qiáng)陰離子 巨孔型 >1,000,000 0–14/0–14 1 0.7 氯化物官能團(tuán)：r–ch2n+（ch3）3 基質(zhì)：聚苯乙烯二乙烯基苯 應(yīng)用：巨孔型ag 1 樹脂。其每克干粉的有效表面面積約23 m2，等價于20% 多孔性。能在有機(jī)溶劑中使用，可用于從水中吸收硅膠，進(jìn)行同工酶分離和hplc 蛋白純化。ag mp–50 樹脂 類型：強(qiáng)陽離子 巨孔型 >1,000,000 0–14/0–14 1.5 0.8 氫官能團(tuán)：r–so3– 基質(zhì)：聚苯乙烯二乙烯基苯 應(yīng)用：巨孔型ag 50w 樹脂。其每克干粉的有效表面積約35 m2，等價于30–35% 多孔性?？捎糜谖誧a2+, mg2+, na+ 和抗生素。ag 4–x4 樹脂 類型：弱陰離子 4% 1,400 <7/0–14 0.8 0.7 自由堿基官能團(tuán)： r–ch2n+h（ch3）2 基質(zhì)：丙烯酸 應(yīng)用：適用于高分子量有機(jī)化合物。二碳形式有利于碳水化合物的去離子作用；自由堿基形式有利于吸收溶液中的無機(jī)酸。ag 501–x8 樹脂 類型：混合床 8% 1,000 0–14/0–14 1.5 0.75 h+ + oh–官能團(tuán)：r–so3– and r–ch2n+（ch3）3 基質(zhì)：聚苯乙烯二乙烯基苯 應(yīng)用：由等量的ag1–x8 和ag 50w–x8 組成，用于水或其它非離子物質(zhì)如尿素、甲酰胺、丙烯酰胺的去離子或脫鹽。ag 501–x8（d）樹脂含有染料，當(dāng)載量用盡時，染料由藍(lán)綠色變?yōu)榻鹕?。ag 11 a8 樹脂 類型：混合床 8% 1,000 0–14/0–14 0.7 0.7 自吸附官能團(tuán)：r–ch2n+（ch3）3 和r–ch2coo– 基質(zhì)：聚苯乙烯二乙烯基苯 應(yīng)用：含配對的陰離子和陽離子交換位點(diǎn)，選擇性的阻滯離子化合物。用于非離子化合物的脘鹽和離子化合物的去離子，如從蛋白中去除sds和從氨基酸中去除無機(jī)鹽。 bio–rex 5 樹脂 類型：弱陰離子 4% 1,400 <7/0–14 1.1 0.7 氯化物官能團(tuán)：r–n+（ch3）2c2h4oh 基質(zhì)：聚苯乙烯二乙烯基苯 應(yīng)用：有機(jī)酸的弱陰離子親和交換劑。可用來去除糖中的酸。bio–rex 70 樹脂 類型：弱陽離子 巨網(wǎng) >1,000,000 5–14/0–14 2.4 0.7 鈉鹽官能團(tuán)：r–coo– 基質(zhì)：丙烯酸 應(yīng)用：適應(yīng)高分子量溶質(zhì)的高載量，樹脂不會使蛋白變性。用于純化和分離多肽和蛋白。bio–rex msz 501 （d） 樹脂 類型：混合床樹脂 8 和10% 1,000 0–14/0–14 1.5 0.72 h+ + oh–官能團(tuán)：r–so3– 和r–ch2n+（ch3）3 基質(zhì)：聚苯乙烯二乙烯基苯 應(yīng)用：微球體大小，可減少壓力下降，增加樹脂載量。用于水和其它非電解質(zhì)的脫鹽。含有染料，當(dāng)載量用盡時，染料由藍(lán)綠色變成金色。chelex 100 樹脂 類型：弱陽離子螯合樹脂 1% 3,500 4–14/0–14 0.4 0.65 鈉鹽，鐵鹽官能團(tuán)：r–ch2n（ch2coo–）2 基質(zhì)：聚苯乙烯二乙烯基苯 應(yīng)用：用于緩沖液和離子試劑的超純化?？汕宄饘傥廴疚锒桓淖兎墙饘匐x子的濃度。鐵鹽形式用于草甘膦濃縮。________________________________________目錄 # 描述ag media 140-1231 ag 1-x2 resin, 500 g, chloride, 50–100 dry mesh, 180–500 µm wet bead 140-1241 ag 1-x2 resin, 500 g, chloride, 100–200 dry mesh, 106–250 µm wet bead 140-1251 ag 1-x2 resin, 500 g, chloride, 200–400 dry mesh, 75–180 µm wet bead 140-1253 ag 1-x2 resin, 500 g, acetate, 200–400 dry mesh, 75–180 µm wet bead 140-1331 ag 1-x4 resin, 500 g, chloride, 50–100 dry mesh, 180–425 µm wet bead 140-1341 ag 1-x4 resin, 500 g, chloride, 100–200 dry mesh, 106–250 µm wet bead 140-1351 ag 1-x4 resin, 500 g, chloride, 200–400 dry mesh, 63–150 µm wet bead 140-1421 ag 1-x8 resin, 500 g, chloride, 20–50 dry mesh, 300–1,180 µm wet bead 140-1422 ag 1-x8 resin, 500 g, hydroxide, 20–50 dry mesh, 300–1,180 µm wet bead 140-1431 ag 1-x8 resin, 500 g, chloride, 50–100 dry mesh, 180–425 µm wet bead 140-1441 ag 1-x8 resin, 500 g, chloride, 100–200 dry mesh, 106–180 µm wet bead 140-1443 ag 1-x8 resin, 500 g, acetate, 100–200 dry mesh, 106–180 µm wet bead 140-1444 ag 1-x8 resin, 500 g, formate, 100–200 dry mesh, 106–180 µm wet bead 140-1451 ag 1-x8 resin, 500 g, chloride, 200–400 dry mesh, 45–106 µm wet bead 140-1453 ag 1-x8 resin, 500 g, acetate, 200–400 dry mesh, 45–106 µm wet bead 140-1454 ag 1-x8 resin, 500 g, formate, 200–400 dry mesh, 45–106 µm wet bead 140-2421 ag 2-x8 resin, 500 g, chloride, 20–50 dry mesh, 300–1,180 µm wet bead 140-2451 ag 2-x8 resin, 500 g, chloride, 200–400 dry mesh, 45–106 µm wet bead 140-4341 ag 4-x4 resin, 500 g, free base, 100–200 dry mesh, 75–150 µm wet bead 141-1831 ag mp-1m resin, 500 g, chloride, 50–100 dry mesh, 150–300 µm wet bead 141-1841 ag mp-1m resin, 500 g, chloride, 100–200 dry mesh, 75–150 µm wet bead 141-1851 ag mp-1m resin, 500 g, chloride, 200–400 dry mesh, 38–75 #&181;m wet bead 142-1231 ag 50w-x2 resin, 500 g, hydrogen, 50–100 dry mesh, 300–1,180 µm wet bead 142-1241 ag 50w-x2 resin, 500 g, hydrogen, 100–200 dry mesh, 106–300 µm wet bead 142-1251 ag 50w-x2 resin, 500 g, hydrogen, 200–400 dry mesh, 75–180 µm wet bead 142-1331 ag 50w-x4 resin, 500 g, hydrogen, 50–100 dry mesh, 180–425 µm wet bead 142-1341 ag 50w-x4 resin, 500 g, hydrogen, 100–200 dry mesh, 106–250 µm wet bead 142-1351 ag 50w-x4 resin, 500 g, hydrogen, 200–400 dry mesh, 63–150 µm wet bead 142-1421 ag 50w-x8 resin, 500 g, hydrogen, 20–50 dry mesh, 300–1,180 µm wet bead 142-1431 ag 50w-x8 resin, 500 g, hydrogen, 50–100 dry mesh, 180–425 µm wet bead 142-1441 ag 50w-x8 resin, 500 g, hydrogen, 100–200 dry mesh, 106–250 µm wet bead 142-1451 ag 50w-x8 resin, 500 g, hydrogen, 200–400 dry mesh, 63–150 µm wet bead 142-1641 ag 50w-x12 resin, 500 g, hydrogen, 100–200 dry mesh, 106–250 µm wet bead 142-1651 ag 50w-x12 resin, 500 g, hydrogen, 200–400 dry mesh, 53–106 µm wet bead 142-6424 ag 501-x8 resin, 500 g, h+ + oh-, 20–50 dry mesh, 300–1,180 µm wet bead 142-6425 ag 501-x8（d） resin, 500 g, h+ + oh-, 20–50 dry mesh, 300–1,180 µm wet bead, dye changes to gold at exchange capacity 142-7834 ag 11 a8 resin, 500 g, self-adsorbed, 50–100 dry mesh, 180–425 µm wet bead 143-0841 ag mp-50 resin, 500 g, hydrogen, 100–200 dry mesh, 75–150 µm wet bead bio-rex media 140-7841 bio-rex 5 resin, 500 g, chloride, 100–200 dry mesh, 75–150 µm wet bead 140-7851 bio-rex 5 resin, 500 g, hydrogen, 200–400 dry mesh, 45–75 µm wet bead 142-5822 bio-rex 70 resin, 500 g, sodium, 20–50 dry mesh, 300–1,180 µm wet bead 142-5832 bio-rex 70 resin, 500 g, sodium, 50–100 dry mesh, 150–300 µm wet bead 142-5842 bio-rex 70 resin, 500 g, sodium, 100–200 dry mesh, 75–150 µm wet bead 142-5852 bio-rex 70 resin, 500 g, sodium, 200–400 dry mesh, 45–75 µm wet bead 142-7425 bio-rex msz 501（d） resin, 500 g, h+ + oh-, 25–35 dry mesh, 500–700 µm wet bead chelex media 142-2822 chelex 100 resin, 500 g, sodium, 50–100 dry mesh, 300–1,180 µm wet bead 142-2825 chelex 100 resin, 100 g, iron, 100–200 dry mesh, 150–300 µm wet bead 142-2832 chelex 100 resin, 500 g, sodium, 100–200 dry mesh, 150–300 µm wet bead 142-2842 chelex 100 resin, 500 g, sodium, 200–400 dry mesh, 75–150 µm wet bead macro–prep 25 s 和25 q 層析介質(zhì)macro–prep 25 s 強(qiáng)陽離子交換載體和macro–prep 25 q 強(qiáng)陰離子 交換載體能在高流速、中壓條件下予以高分辨率分離。兩種層析介質(zhì)都 具有50μm macro–prep high q 和high s 層析介質(zhì)相同的特性如：剛性、 大孔性和疏水性，但25μm 微球體能提供更高的分辨率。優(yōu)點(diǎn)包括： • 無論是實(shí)驗(yàn)室研究還是小試規(guī)模的分離，都能獲得zui大樣品回收率和純度 • 減少實(shí)驗(yàn)操作步驟 • 去除蛋白變體等難以處理的污染物，減少這類物質(zhì)在終產(chǎn)品中再次鑒定的步驟 hevea （rubber plant）胞液的銨分離份用macro–prep 25q （11 x 21 cm） 層析柱分離。緩沖液a, 50 mm tris, ph 8.3；緩沖液b, 50 mm tris + 1 m nacl, ph 8.3；流速=5ml/min。macro–prep 離子交換介質(zhì)滿足了分析型、半制備型和工業(yè)化 應(yīng)用的要求。它是剛性的的大孔疏水介質(zhì)，具有很好的載量、 分辨率和通量，且化學(xué)和機(jī)械性能穩(wěn)定。macro–prep 層 析介質(zhì)在低壓和中壓條件下也能運(yùn)行良好，即使ph 和 離子強(qiáng)度有變化，體積變化也很小。它可以用naoh 和促溶劑消毒，也可高壓滅菌。能提供穩(wěn)定、重復(fù)的 結(jié)果。macro–prep 離子交換介質(zhì)特性： • 對生物分子具有高載量 • 對復(fù)雜生物混合物具有高分辨率 • 剛性的多聚體基質(zhì)可承受中壓和高流速 • 親水基質(zhì)減少非特異性結(jié)合 • 巨孔顆粒提高離子位點(diǎn)的結(jié)合 • 化學(xué)、機(jī)械和熱穩(wěn)定性 目錄 # 描述macro-prep cm media 156-0070 macro-prep cm support, 100 ml 156-0071 macro-prep cm support, 500 ml 156-0072 macro-prep cm support, 5 l 156-0073 macro-prep cm support, 10 l 158-0070 macro-prep cm support, 25 ml macro-prep deae media 156-0020 macro-prep deae support, 100 ml 156-0021 macro-prep deae support, 500 ml 156-0022 macro-prep deae support, 5 l 156-0023 macro-prep deae support, 10 l 158-0020 macro-prep deae support, 25 ml macro-prep high q media 156-0040 macro-prep high q support, 100 ml 156-0041 macro-prep high q support, 500 ml 156-0042 macro-prep high q support, 5 l 156-0043 macro-prep high q support, 10 l 158-0040 macro-prep high q support, 25 ml macro-prep high s media 156-0030 macro-prep high s support, 100 ml 156-0031 macro-prep high s support, 500 ml 156-0032 macro-prep high s support, 5 l 156-0033 macro-prep high s support, 10 l 158-0030 macro-prep high s support, 25 ml unosphere 離子交換介質(zhì)是為滿足生物制藥界對具有 更高生產(chǎn)能力層析介質(zhì)的需要而設(shè)計(jì)的。unosphere 是新 一代的親水聚丙烯酰胺多聚載體，由一步聚合而成，具有高 結(jié)合能力和低背壓的特點(diǎn)，從而可提供更高的生產(chǎn)能力。一 步聚合法生產(chǎn)的載體具有*的品質(zhì)和批間穩(wěn)定性。 unosphere 離子交換介質(zhì)特性包括：• 適合在背壓2 bar，線性速度1,200 cm/hr 下運(yùn)行 • 在1m 的naoh 中，仍能保持10,000 小時穩(wěn)定性 • 有效吸附樣品中的生物醫(yī)藥分子 • 孔隙大，技術(shù)線性流速很高時也產(chǎn)生超高的結(jié)合能力 • 親水多聚微球體，良好得機(jī)械穩(wěn)定性和適應(yīng)低背壓 • 耐用多聚體，可以多次循環(huán)清洗 • 產(chǎn)生足夠生物制藥生產(chǎn)所需的層析量 • 全面提供藥品管理申報的幫助 unosphere 介質(zhì)的結(jié)合特性與背壓特性。柱大小，1.1x 20 cm。上圖， 2 mg/ml 人igg上樣于unosphere s 柱；緩沖液，50 mm 醋酸鈉，ph 5.0。下 圖， 5 mg/ml bsa（溶于10 mm tris，ph 8.5）上樣于unosphere q 柱。紅 線，背壓；藍(lán)線，10% 流穿載量。目錄 # 描述unosphere q anion exchange support 156-0101 unosphere q support, 25 ml 156-0103 unosphere q support, 100 ml 156-0105 unosphere q support, 500 ml 156-0107 unosphere q support, 10 l unosphere s cation exchange support 156-0111 unosphere s support, 25 ml 156-0113 unosphere s support, 100 ml 156-0115 unosphere s support, 500 ml 156-0117 unosphere s support, 10 l also available in biopharmaceutical manufacturing quantitiespoly–prep層析柱是高9 cm，內(nèi)徑0.8 x 4 cm的圓錐形聚丙烯柱， 可填裝多達(dá)2 ml層析介質(zhì)。內(nèi)置儲液器可保存10 ml洗脫液或樣品。應(yīng) 用于樣品制備和小規(guī)模層析，包括放射性同位元素和其它一次性產(chǎn)品 的應(yīng)用。poly–prep層析柱可高壓滅菌并能保持細(xì)小顆粒。目錄 # 描述poly-prep columns 731-1550 poly-prep chromatography columns, empty, includes end caps and tip closures, 50 731-1553 poly-prep chromatography columns, empty, includes end caps and tip closures, 1,000 accessories 731-1555 poly-prep column stack cap, 50 731-7005 poly column rack, 20-place, with removable tube rack 731-8232 female luer plugs, 25 732-8102 2-way stopcocks, female luer to male luer, 10 poly–prep層析柱是高9 cm，內(nèi)徑0.8 x 4 cm的圓錐形聚丙烯柱， 可填裝多達(dá)2 ml層析介質(zhì)。內(nèi)置儲液器可保存10 ml洗脫液或樣品。應(yīng) 用于樣品制備和小規(guī)模層析，包括放射性同位元素和其它一次性產(chǎn)品 的應(yīng)用。poly–prep層析柱可高壓滅菌并能保持細(xì)小顆粒。目錄 # 描述econo-pac columns 732-1010 econo-pac chromatography columns, empty, includes upper bed supports, end caps, tip closures, 50 732-1011 econo-pac chromatography columns, empty, includes upper bed supports, end caps, tip closures, 500 accessories 731-7005 poly column rack, 20-place, with removable tube rack 731-8232 female luer plugs, 25 732-8102 2-way stopcocks, female luer to male luer, 10 738-0019 econo-pac flow adaptor, 1.5 cm column id 儲液器有500 ml和1 l兩種規(guī)格，與econo–column層析柱（0.5、0.7、 1.0或1.5 cm 內(nèi)徑）相匹配。如需要穩(wěn)壓的儲液器，只需關(guān)閉與儲液器連 有玻璃管的活塞頂帽?？梢苿由仙w帶有凸形luer–lok接頭。目錄 # 描述737-9112 econo-column reservoir, 500 ml 737-9113 econo-column reservoir, 1 l 說明： econo–column 漏斗的材料是耐用聚丙烯，是裝柱、稀釋樣品上樣 或大體積緩沖液輸送的理想選擇。它可與econo–column層析柱（直徑 2.5 cm）、護(hù)套econo–column層析柱、poly–prep層析柱和econo–pac 層析柱密封結(jié)合。目錄 # 描述731-0003 econo-column funnels, 250 ml, 5 液流轉(zhuǎn)換接頭可用于去掉凝膠柱床上部的死體積，并保 護(hù)柱床在加樣過程中免受干擾，有效地提高了層析柱性能。 液流轉(zhuǎn)換接頭直接把緩沖液和樣品傳送到柱床頂部，提高了 分辨率。建議將該接頭與各種連有泵的低壓柱、biologic lp 系統(tǒng)或其它低壓系統(tǒng)配合使用。econo–column 液流轉(zhuǎn)換接頭，可與econo–column 層析柱（1.0、1.5、2.5 和5.0 cm）、護(hù)套econo–column 層 析柱和econo–pac 層析柱匹配。目錄 # 描述flow adaptors 738-0014 flow adaptor, 1.0 cm column id, 1–7 cm functional length 738-0015 flow adaptor, 1.0 cm column id, 1–14 cm functional length 738-0016 flow adaptor, 1.5 cm column id, 1–14 cm functional length 738-0017 flow adaptor, 2.5 cm column id, 1–14 cm functional length 738-0018 flow adaptor, 5.0 cm column id, 1–14 cm functional length （does not include cam mechanism） 738-0019 econo-pac flow adaptor, 1.5 cm column id flow adaptor replacement parts 738-0022 flow adaptor maintenance kit, for 5.0 cm flow adaptor, includes 2 bed supports and 2 o-rings 738-0024 flow adaptor maintenance kit, for 1.0 cm adaptor with cam mechanism, includes 10 bed supports and 5 o-rings 738-0025 flow adaptor maintenance kit, for 1.5 cm adaptor with cam mechanism, includes 10 bed supports and 5 o-rings 738-0027 flow adaptor maintenance kit, for 2.5 cm adaptor with cam mechanism, includes 10 bed supports and 5 o-rings econo–column 層析柱是優(yōu)質(zhì)而又實(shí)惠的低壓層析柱。擁有各種不同的尺寸規(guī)格長度范圍5–170cm，直徑0.5– 5cm。柱底部的多孔聚合物柱床撐板能保留細(xì)小顆粒，透明的聚丙烯末端接頭便于監(jiān)測。econo–column 層析柱可 高壓滅菌并可在壓力<1 bar （14.7 psi） 下操作。econo–column 層析柱與econo–column 漏斗和econo–column液流 轉(zhuǎn)換接頭匹配。目錄 # 描述econo-column chromatography columns 737-0506 econo-column chromatography columns, 0.5 x 5 cm, 1 ml, 5 737-0507 econo-column chromatography columns, 0.5 x 5 cm, 1 ml, 2 737-0511 econo-column chromatography columns, 0.5 x 10 cm, 2 ml, 5 737-0512 econo-column chromatography columns, 0.5 x 10 cm, 2 ml, 2 737-0516 econo-column chromatography columns, 0.5 x 15 cm, 3 ml, 5 737-0517 econo-column chromatography columns, 0.5 x 15 cm, 3 ml, 2 737-0521 econo-column chromatography columns, 0.5 x 20 cm, 4 ml, 5 737-0522 econo-column chromatography columns, 0.5 x 20 cm, 4 ml, 2 737-0706 econo-column chromatography columns, 0.7 x 5 cm, 2 ml, 5 737-0707 econo-column chromatography columns, 0.7 x 5 cm, 2 ml, 2 737-0711 econo-column chromatography columns, 0.7 x 10 cm, 4 ml, 5 737-0712 econo-column chromatography columns, 0.7 x 10 cm, 4 ml, 2 737-0716 econo-column chromatography columns, 0.7 x 15 cm, 6 ml, 5 737-0717 econo-column chromatography columns, 0.7 x 15 cm, 6 ml, 2 737-0721 econo-column chromatography columns, 0.7 x 20 cm, 8 ml, 5 737-0722 econo-column chromatography columns, 0.7 x 20 cm, 8 ml, 2 737-0731 econo-column chromatography columns, 0.7 x 30 cm, 12 ml, 5 737-0732 econo-column chromatography columns, 0.7 x 30 cm, 12 ml, 2 737-0751 econo-column chromatography columns, 0.7 x 50 cm, 20 ml, 5 737-0752 econo-column chromatography columns, 0.7 x 50 cm, 20 ml, 2 737-1006 econo-column chromatography columns, 1.0 x 5 cm, 4 ml, 5 737-1007 econo-column chromatography columns, 1.0 x 5 cm, 4 ml, 2 737-1011 econo-column chromatography columns, 1.0 x 10 cm, 8 ml, 5 737-1012 econo-column chromatography columns, 1.0 x 10 cm, 8 ml, 2 737-1021 econo-column chromatography columns, 1.0 x 20 cm, 16 ml, 5 737-1022 econo-column chromatography columns, 1.0 x 20 cm, 16 ml, 2 737-1031 econo-column chromatography columns, 1.0 x 30 cm, 24 ml, 5 737-1032 econo-column chromatography columns, 1.0 x 30 cm, 24 ml, 2 737-1051 econo-column chromatography columns, 1.0 x 50 cm, 40 ml, 5 737-1052 econo-column chromatography columns, 1.0 x 50 cm, 40 ml, 2 737-1091 econo-column chromatography columns, 1.0 x 100 cm, 79 ml, 2 737-1093 econo-column chromatography columns, 1.0 x 120 cm, 103 ml, 2 737-1506 econo-column chromatography columns, 1.5 x 5 cm, 9 ml, 5 737-1507 econo-column chromatography columns, 1.5 x 5 cm, 9 ml, 2 737-1511 econo-column chromatography columns, 1.5 x 10 cm, 18 ml, 5 737-1512 econo-column chromatography columns, 1.5 x 10 cm, 18 ml, 2 737-1516 econo-column chromatography columns, 1.5 x 15 cm, 27 ml, 5 737-1517 econo-column chromatography columns, 1.5 x 15 cm, 27 ml, 2 737-1521 econo-column chromatography columns, 1.5 x 20 cm, 35 ml, 5 737-1522 econo-column chromatography columns, 1.5 x 20 cm, 35 ml, 2 737-1531 econo-column chromatography columns, 1.5 x 30 cm, 53 ml, 5 737-1532 econo-column chromatography columns, 1.5 x 30 cm, 53 ml, 2 737-1551 econo-column chromatography columns, 1.5 x 50 cm, 89 ml, 5 737-1552 econo-column chromatography columns, 1.5 x 50 cm, 89 ml, 2 737-1576 econo-column chromatography columns, 1.5 x 75 cm, 124 ml, 2 737-1591 econo-column chromatography columns, 1.5 x 100 cm, 177 ml, 2 737-1593 econo-column chromatography columns, 1.5 x 120 cm, 230 ml, 2 737-1598 econo-column chromatography columns, 1.5 x 170 cm, 301 ml, 2 737-2506 econo-column chromatography columns, 2.5 x 5 cm, 25 ml, 5 737-2507 econo-column chromatography columns, 2.5 x 5 cm, 25 ml, 2 737-2511 econo-column chromatography columns, 2.5 x 10 cm, 49 ml, 5 737-2512 econo-column chromatography columns, 2.5 x 10 cm, 49 ml, 2 737-2521 econo-column chromatography columns, 2.5 x 20 cm, 98 ml, 5 737-2522 econo-column chromatography columns, 2.5 x 20 cm, 98 ml, 2 737-2531 econo-column chromatography columns, 2.5 x 30 cm, 147 ml, 5 737-2532 econo-column chromatography columns, 2.5 x 30 cm, 147 ml, 2 737-2551 econo-column chromatography columns, 2.5 x 50 cm, 246 ml, 2 737-2576 econo-column chromatography columns, 2.5 x 75 cm, 344 ml, 2 737-2591 econo-column chromatography columns, 2.5 x 100 cm, 491 ml, 2 737-2593 econo-column chromatography columns, 2.5 x 120 cm, 589 ml, 2 737-5011 econo-column chromatography column, 5.0 x 10 cm, 196 ml, 1 737-5021 econo-column chromatography column, 5.0 x 20 cm, 393 ml, 1 737-5031 econo-column chromatography column, 5.0 x 30 cm, 589 ml, 1 737-5051 econo-column chromatography column, 5.0 x 50 cm, 982 ml, 1 737-5071 econo-column chromatography column, 5.0 x 70 cm, 1,374 ml, 1 737-6108 econo-column standard jacketed column, 0.7 x 15 cm, 6 ml 737-6116 econo-column standard jacketed column, 1.0 x 15 cm, 12 ml 737-6131 econo-column standard jacketed column, 1.0 x 30 cm, 25 ml 737-6151 econo-column standard jacketed column, 1.5 x 50 cm, 89 ml 737-6201 econo-column open-ended jacketed chromatography column, 1 x 30 cm, includes 2 flow adaptors for dna hydroxyapatite chromatography, 25 ml econo-column se-lection packs 737-6601 econo-column se-lection pack a, includes 7 columns, 1 each of 0.7 x 10, 20, and 30 cm; 1.5 x 30 and 50 cm; 2.5 x 20 and 50 cm 737-6607 econo-column se-lection pack b, includes 6 columns, 1 each of 1.0 x 20, 30, and 50 cm; 1.5 x 20, 30, and 50 cm bio–scale mt 空柱可填裝你所選擇的介質(zhì)。這些層析柱能為大多數(shù)層析提供*的分辨率。bio– scale mt 層析柱可精確上樣，并提供高分辨率分離所需的低死體積。四種不同規(guī)格的柱（2、5、10、和 20ml）可使你輕松完成各級分離和純化操作。優(yōu)化的設(shè)計(jì)便于裝柱、調(diào)高、上樣和平衡。bio–scale mt 層析柱可方便地與biologic 系統(tǒng)或任何中高壓層析系統(tǒng)配套使用。目錄 # 描述bio-scale mt columns 751-0081 bio-scale mt2 column, 7 x 52 mm, 1.9–2.3 ml, 1,000 psi pressure limit 751-0083 bio-scale mt5 column, 10 x 64 mm, 4.6 to 5.7 ml, 750 psi pressure limit 751-0085 bio-scale mt10 column, 12 x 88 mm, 9.5 to 11.3 ml, 600 psi pressure limit 751-0087 bio-scale mt20 column, 15 x 113 mm, 19.4 to 21.9 ml, 500 psi pressure limit accessories 750-0554 1/16" od （1.6 mm） post-pump fittings, includes delrin nut, ferrules, lock ring, 10 sets 750-0567 uno m6 fittings kit, includes 2 nuts and 4 ferrules to connect uno column to an fplc system 750-0568 u0-32 fittings kit, includes 2 nuts and 4 ferrules to connect uno column to an hplc system 751-0091 bio-scale 2 replacement parts kit, includes 5 frits, 5 distribution screens, 2 0-rings, 1 frit remover 751-0093 bio-scale 5 replacement parts kit, includes 5 frits, 5 distribution screens, 2 o-rings, 1 frit remover 751-0095 bio-scale 10 replacement parts kit, includes 5 frits, 5 distribution screens, 2 o-rings, 1 frit remover 751-0097 bio-scale 20 replacement parts kit, includes 5 frits, 5 distribution screens, 2 o-rings, 1 frit remover bio-spin and micro bio-spin columns clean up dna as well as protein samples quickly. filled with specially sized bio-gel p gels, these columns are ideal for quickly and inexpensively removing unincorporated dye terminators from automated sequencing reactions. bio-spin columns are shipped fully hydrated and are ready to use. the simple procedure takes just 10 minutesguide to bio-spin columnsbio-spin 6 microbio-spin 6 bio-spin 30 microbio-spin 30packed support special grade bio-gel p-6 gel special grade bio-gel p-6 gel special grade bio-gel p-30 gel special grade bio-gel p-30 gelequilibration buffer ssc buffer＊ 10 mm tris, ph 7.4 or ssc buffer＊ ssc buffer＊ 10 mm tris, ph 7.4 or ssc buffer＊applications desalting and buffer exchange desalting and buffer exchange desalting; nucleotide and small molecule removal dna sequencing reaction mixtures （tris）, and small molecule removalbed volume 1.1 ml 0.7 ml 1.1 ml 0.7 mlretention and recovery 90% recovery of 20 bases or bp, 99% retention of salts 90% recovery of 20 bases or bp, 99% retention of salts 95% recovery of 22 bases or bp, 98% retention of ddntps 95% recovery of 22 bases or bp, 98% retention of ddntpsmw exclusion limit, globular proteins 6,000 6,000 40,000 40,000sample volume 50–100 µl 10–75 µl 50–100 µl 10–750 µlcentrifuge type swinging bucket microcentrifuge swinging bucket microcentrifugeautoclavability yes yes yes yes＊ 150 mm nacl, 17.5 mm sodium citrate, ph 7.0目錄 # 描述bio-spin columns with bio-gel p-30 gel 732-6006 bio-spin 30 columns, includes 25 columns in ssc buffer, 50 collection tubes bio-spin columns with bio-gel p-30 in tris buffer 732-6231 bio-spin 30 columns, includes 25 columns in tris buffer, 50 collection tubes 732-6232 bio-spin 30 columns, includes 100 columns in tris buffer, 200 collection tubes bio-spin columns with bio-gel p-6 gel in ssc buffer 732-6002 bio-spin 6 columns, includes 25 columns in ssc buffer, 50 collection tubes 732-6213 bio-spin 6 columns, includes 1,000 columns in ssc buffer, 2,000 collection tubes bio-spin columns with bio-gel p-6 in tris buffer in ssc buffer 732-6227 bio-spin 6 columns, includes 25 columns in tris buffer, 50 collection tubes 732-6228 bio-spin 6 columns, includes 100 columns in tris buffer, 200 collection tubes micro bio-spin columns with bio-gel p-30 gel in ssc buffer 732-6202 micro bio-spin 30 columns, includes 25 columns in ssc buffer, 50 collection tubes 732-6203 micro bio-spin 30 columns, includes 100 columns in ssc buffer, 200 collection tubes 732-6206 micro bio-spin 30 columns, includes 1,000 columns in ssc buffer, 2,000 collection tubes micro bio-spin columns with bio-gel p-30 gel in tris buffer 732-6223 micro bio-spin 30 columns, includes 25 columns in tris buffer, 50 collection tubes 732-6224 micro bio-spin 30 columns, includes 100 columns in tris buffer, 200 collection tubes 732-6226 micro bio-spin 30 columns, includes 1,000 columns in tris buffer, 2,000 collection tubes 732-6250 micro bio-spin 30 columns, includes 25 columns in tris buffer, 50 collection tubes, rnase-free 732-6251 micro bio-spin 30 columns, includes 100 columns in tris buffer, 200 collection tubes, rnase-free micro bio-spin columns with bio-gel p-6 gel in ssc buffer 732-6200 micro bio-spin 6 columns, includes 25 columns in ssc buffer, 50 collection tubes 732-6201 micro bio-spin 6 columns, includes 100 columns in ssc buffer, 200 collection tubes 732-6205 micro bio-spin 6 columns, includes 1,000 columns in ssc buffer, 2,000 collection tubes micro bio-spin columns with bio-gel p-6 gel in tris buffer 732-6221 micro bio-spin 6 columns, includes 25 columns in tris buffer, 50 collection tubes 732-6222 micro bio-spin 6 columns, includes 100 columns in tris buffer, 200 collection tubes 732-6225 micro bio-spin 6 columns, includes 1,000 columns in tris buffer, 2,000 collection tubes 目錄 # 描述731-1555 poly-prep column stack cap, 50 731-6211 poly-prep columns, prefilled with ag 1-x8 resin, 100–200 mesh, 50 731-6212 poly-prep columns, prefilled with ag 1-x8 resin, 200–400 mesh, 50 731-6213 poly-prep columns, prefilled with ag 50w-x8 resin, 100–200 mesh, 50 731-6214 poly-prep columns, prefilled with ag 50w-x8 resin, 200–400 mesh, 50 731-6221 poly-prep columns, prefilled with ag 1-x8 resin, 200–400 mesh, 50 預(yù)裝poly–prep 柱，用于重力流層析樣品制備和其它小規(guī)模試驗(yàn)。 0.8 x 4 cm 聚丙烯層析柱帶有刻度。每個柱均填裝有2 ml 的標(biāo)準(zhǔn)床體 積ag 離子交換樹脂，并內(nèi)置一個10ml 儲液器。同時提供可與泵、 儲液器或其他系列層析柱連接的poly–prep 疊合蓋。預(yù)裝econo–pac 柱用于重力流層析，可進(jìn)行快速而簡單的脫鹽以及簡便的抗體純化。預(yù)裝 econo–pac 柱包括一個上部濾板、折斷式末梢、層析柱刻度標(biāo)記和30ml 柱容量。使用液流轉(zhuǎn)換接頭 可改變柱的功能。目錄 # 描述732-2010 econo-pac 10dg desalting columns, 30 per box 732-2020 econo-pac protein a kit, 1 x 2 ml protein a column, 1 x 10 ml 10dg column, buffers 732-2022 econo-pac protein a columns, prefilled with affi-gel protein a gel, 5 732-2027 econo-pac serum igg purification kit, 5 x 5 ml deae affi-gel blue columns, 1 x 10 ml 10dg column, buffers 738-0019 econo-pac flow adaptor, 1.5 cm column id uno monolith 離子交換層析柱含有*的連續(xù)床 基質(zhì)，能在高流速下分離生物分子而不降低分辨率或載 量。uno 層析柱集中了傳統(tǒng)介質(zhì)的*特性（如生物 活性高、背壓低、非特異性結(jié)合低和ph 2–12 范圍內(nèi)穩(wěn) 定）以及與連續(xù)床技術(shù)相同的速度、分辨率和載量。 uno 層析柱在低背壓條件下可與biologic duoflow 系統(tǒng)或任何其它中高壓層析系統(tǒng)配合使用。連續(xù)床技術(shù)中，新式的單體和離子化合物直接在 層析柱中聚合，形成緊密的均質(zhì)節(jié)結(jié)網(wǎng)狀物。所產(chǎn)生的 剛性基質(zhì)含有互連通道，足以在低背壓下提供快速液流。 連續(xù)床基質(zhì)能為層析柱提供良好的分辨率、速度、載量 和高超的生物分子分離能力。均質(zhì)無孔連續(xù)床基質(zhì)能高 速地把大量蛋白和洗脫液傳遞給離子官能團(tuán)，減少了條 帶變寬，并提供*的蛋白峰分辨率。*的uno 基質(zhì)具有大多數(shù)微球體介質(zhì)不同的 特性：• 高流速下也有較高的分辨率 • 高流速下也能保持高載量，這是由于生物分子可通過互連通道*接觸含有離子官能團(tuán)的緊密結(jié)節(jié)網(wǎng)狀物。1 ml 的uno 層析柱可在3 分鐘內(nèi)完成高分辨率分離 • *的品質(zhì)和批次間重復(fù)性。均質(zhì)連續(xù)床基質(zhì)具有防裂特性，使用壽命長 • 具有強(qiáng)陰離子（q）和強(qiáng)陽離子（s）交換兩種形式 有關(guān)層析柱選擇的更多信息，請索取資料# 2116。 phosphoinositide–3–kinase 純化。 緩沖液a, 40 mm tea, ph 7.6 緩沖液b, 緩沖液a + 1 m kcl, ph 7.6 梯度0–35% b , 20 ml 流速2.5 ml/min以上數(shù)據(jù)由dr a couvillon, beth israeldeaconess medical center, boston, ma usa 提供。掃描電子顯微圖顯示連續(xù)床基質(zhì)的結(jié)構(gòu)（1,950 x 放大倍率）。 注意聚合物節(jié)結(jié)的節(jié)結(jié)狀結(jié)構(gòu)和它們之間的大通道。uno 玻璃柱uno 玻璃柱提供高分辨率的生物分子分離。透明 玻璃管便于察看柱床和層析故障監(jiān)測。3 種不同的尺寸 配合不同的純化操作要求。uno 替換柱uno 替換柱提供簡便的柱床替換，經(jīng)濟(jì)實(shí)用。uno 精加工peek 層析柱uno 精加工層析柱是一種后期純化工具，可以從 少量樣品中獲得zui高的分辨率和回收率。只需一步操作 即能同時完成稀釋樣品的純化和濃縮。目錄 # 描述uno glass columns 720-0001 uno q1 column, 7 x 35 mm, 1.3 ml 720-0003 uno q6 column, 12 x 53 mm, 6 ml 720-0005 uno q12 column, 15 x 68 mm, 12 ml 720-0021 uno s1 column, 7 x 35 mm, 1.3 ml 720-0023 uno s6 column, 12 x 53 mm, 6 ml 720-0025 uno s12 column, 15 x 68 mm, 12 ml uno polishing peek columns 720-0009 uno q polishing column, 4.6 x 10 mm, 0.16 ml 720-0029 uno s polishing column, 4.6 x 10 mm, 0.16 ml uno replacement columns 720-0011 uno q1r column, 7 x 35 mm, 1.3 ml 720-0013 uno q6r column, 12 x 53 mm, 6 ml 720-0015 uno q12r column, 12 ml 720-0031 uno s1r column, 7 x 35 mm, 1.3 ml 720-0033 uno s6r column, 12 x 53 mm, 6 ml 720-0035 uno s12r column, 12 ml fittings 750-0554 1/16" od （1.6 mm） post-pump fittings, includes delrin nut, ferrules, lock ring, 10 sets 750-0567 uno m6 fittings kit, includes 2 nuts and 4 ferrules to connect uno column to an fplc system 750-0568 u0-32 fittings kit, includes 2 nuts and 4 ferrules to connect uno column to an hplc system酵母菌酶分離在1 ml/min（a）和5 ml/min（b）流速下的比較。 緩沖液，20 mm tris, ph 8.2; 梯度，0–1 m nacl , 20 ml.bio–scale cht i 型層析柱填裝了10 μm i 型cht 陶瓷羥基磷灰石介質(zhì)，這種介質(zhì)對堿性蛋白親和力高， 對酸性蛋白的親和力較低。這些預(yù)裝柱為分析型、半制 備型等各種中壓應(yīng)用提供快速而可重復(fù)的高分辨率分 離，它們可與biologic 系統(tǒng)或任何中高壓層析系統(tǒng)配 合使用。bio–scale cht i 型柱頂層介質(zhì)更換試劑盒在層析過程中，如果常規(guī)的消毒步驟不能消除柱床 頂部污染，i 型bio–scale cht 柱頂層介質(zhì)更換試劑盒 可去除部分柱床并更換新鮮載體。該試劑盒含有1ml 載 體、濾板和每種柱直徑的沉積擋板。bio–scale cht20–i 層析柱應(yīng)用于小鼠腹水中純化ig m條件 樣品 2.3 ml （2:1 稀釋）流速 4.6 ml/min緩沖液 a, 10 mm 磷酸鈉緩沖液, ph 6.8b, 500 mm 磷酸緩沖液, ph 6.8目錄 # 描述bio-scale columns 751-0021 bio-scale cht2-i column, 7 x 52 mm, 2 ml bed volume, 20 mg protein capacity, flow rate 0.5–3.0 ml/min, 1,000 psi 751-0023 bio-scale cht5-i column, 10 x 64 mm, 5 ml bed volume, 50 mg protein capacity, flow rate 0.5–5.0 ml/min, 750 psi 751-0025 bio-scale cht10-i column, 12 x 88 mm, 10 ml bed volume, 100 mg protein capacity, flow rate 0.5–7.0 ml/min, 600 psi 751-0027 bio-scale cht20-i column, 15 x 113 mm, 20 ml bed volume, 200 mg protein capacity, flow rate 0.5–10.0 ml/min, 500 psi 751-0029 cht-i top-off support kit, 1 ml fittings 750-0554 1/16" od （1.6 mm） post-pump fittings, includes delrin nut, ferrules, lock ring, 10 sets 750-0567 uno m6 fittings kit, includes 2 nuts and 4 ferrules to connect uno column to an fplc system 750-0568 u0-32 fittings kit, includes 2 nuts and 4 ferrules to connect uno column to an hplc system bio–sil 和bio–silect 層析柱是以硅材料為基質(zhì)，用 于樣品成分分離、脫鹽或緩沖液置換和分子量或分子 常數(shù)估算。硅材料的分子量排阻層析柱應(yīng)用于分離ph 2–8 的多肽、蛋 白和核酸。分析柱有不銹鋼（bio–sil）和生物兼容的peek塑料（bio– silect）兩種。bio–sil 和bio–silect sec 柱填裝了5μm 親水結(jié)合相硅，蛋白 載量0.01–1.5 mg。為了分離洗脫峰較接近的蛋白，獲得更高的分辨率，可 串聯(lián)使用2 個300 mm sec 柱、工作壓>1000 psi 的系列層析系統(tǒng)。該層析柱確保在70°c 下的熱穩(wěn)定性以及化學(xué)穩(wěn)定性。介質(zhì)在甲醇、 乙醇、氰化甲烷、dmso 和thf 等有機(jī)溶劑中穩(wěn)定。更多信息，請索取資料# 1737。 用bio–silect 125–5（左）；bio–silect sec 250–5（中）； bio–silect 400–5（右）柱分離凝膠過濾標(biāo)準(zhǔn)品（貨號#151–1901）。 峰：1，甲狀腺球蛋白，670 kd；2，igg，158 kd；3，卵白蛋白，44 kd；4，肌球蛋白，17 kd；5，維生素b12, 1.35 kd分離zona pellucida糖蛋白。感謝dr jeffrey d bleil, the scripps research institute, la jolla, ca usa 提供數(shù)據(jù)。bio–sil sec 250 層析柱分離igg 片段。感謝jay e persselin, wadsworth va medical center, los angeles, ca usa.提供數(shù)據(jù)。 目錄 # 描述bio-sil sec columns 125-0060 bio-sil sec 125-5 column, 300 x 7.8 mm 125-0062 bio-sil sec 250-5 column, 300 x 7.8 mm 125-0064 bio-sil sec 400-5 column, 300 x 7.8 mm 125-0072 bio-sil sec 125 guard, 80 x 7.8 mm 125-0073 bio-sil sec 250 guard, 80 x 7.8 mm 125-0074 bio-sil sec 400 guard, 80 x 7.8 mm bio-silect sec columns 125-0475 bio-silect sec 125-5 column, 300 x 7.8 mm 125-0476 bio-silect sec 250-5 column, 300 x 7.8 mm 125-0477 bio-silect sec 400-5 column, 300 x 7.8 mm 125-0478 bio-silect sec 125 guard, 50 x 7.8 mm 125-0479 bio-silect sec 250 guard, 50 x 7.8 mm 125-0480 bio-silect sec 400 guard, 50 x 7.8 mm 由聚苯乙烯二乙烯苯樹脂填裝而成的 aminex hplc 柱，具有良好的高壓及ph 穩(wěn)定 性、高柱效和選擇性。aminex hplc 柱通過離 子調(diào)節(jié)分配層析技術(shù)分離混合物，這種技術(shù)是 根據(jù)多種不同的化學(xué)特性分離分子。離子調(diào)節(jié) 分配層析技術(shù)的分離機(jī)制包括離子排阻、離子 交換、正相和反相分配、分子量排阻和配體交換。 根據(jù)層析的物理和化學(xué)特性，可選擇使用一個或多 個分離機(jī)制。aminex 柱是工業(yè)界普遍使用的，用來分 析碳水化合物、有機(jī)酸、有機(jī)堿和其它包括肽和核酸的 有機(jī)小分子的標(biāo)準(zhǔn)產(chǎn)品。用aminex 柱分離復(fù)雜混合物需 要使用簡單等流相（常用水），并要求精確控溫。研究過程中，樣品可重復(fù)的保留量和分辨率是至關(guān) 重要的。不同批次間及不同柱間的測試證明aminex 柱是 值得信賴的產(chǎn)品。aminex 柱的使用壽命長，并能保持上百次試驗(yàn)的可 重復(fù)性。耐用的層析柱避免了頻繁更換柱引起的數(shù)據(jù)變 化和高成本。aminex 柱應(yīng)用實(shí)例• 木漿水解產(chǎn)物分析 • 玉米汁成分分離 • 大鼠肝流出液層析圖譜 • 從cheddar 奶酪中分離有機(jī)酸 aminex 指南是食品、飲料和生物技 術(shù)產(chǎn)業(yè)界的指南，可分析生物加工 過程中產(chǎn)生的有機(jī)酸、酒、糖、胺、 細(xì)胞代謝物和其它中間化合物。 更多信息，請索取資料# 1928。applications particlesize （μm） ionicform cross-linkage % phrangecarbohydrate analysis columns125-0143 aminex hpx-87n column, 300 x 7.8 mm beet sugars 9 sodium 8 5–9125-0142 aminex hpx-87k column, 300 x 7.8 mm molasses, corn syrup 9 potassium 8 5–9