伯樂雙染蛋白maker現(xiàn)貨161-0374
Applications and Uses
Choose Precision Plus Protein Dual Color standards for SDS-PAGE:
- Monitoring electrophoretic separation
- Molecular weight sizing
- Monitoring blot transfer efficiency with easy orientation via multicolor standards
- Fluorescent blot detection， with blue bands visualized in the red wavelength range and pink bands in the green wavelength (prepare a 1:10 dilution of the Dual Color standard and load 5 µl per well)
伯樂雙染蛋白maker現(xiàn)貨161-0374
More Information
Loading buffer:
30% (w/v) glycerol， 2% SDS， 62.5 mM Tris， pH 6.8， 50 mM DTT， 5 mM EDTA， 0.02% NaN3， 0.01% bromophenol blue
- Description
- Specifications
| MW size range， kD | 10–250 | |
| Stain type | Dual Color | |
| Volume， µl | 500 | |
| Total number of applications | 50 | |
| Shelf life at –20°C， months | 12 | |
| Loading volume on mini gels， µl | for Coomassie staining， 10 | |
| Loading volume on mini gels， µl | for fluorescent blot detection， 5 of 1:10 dilution | |
| Application Type | ||
| Monitoring electrophoretic separation for SDS-PAGE gels | Yes | |
| Molecular weight sizing on SDS-PAGE gels | Yes | |
| Monitoring blot transfer efficiency | Yes | |
| Fluorescence blots | Yes | |
| Molecular weight sizing on | N/A | |
| Loading Volume on Mini Gels | ||
| For Coomassie staining， µl | N/A | |
| For stain-free gels， µ | N/A | |
| For silver staining， µl | N/A | |
| For SYPRO Ruby staining， µl | N/A | |
| For blot development， µl | N/A | |
| StrepTactin conjugate working dilutions for colorimetric blot development | N/A | |
| StrepTactin conjugate working dilutions for chemiluminescence blot development | N/A |
The Precision Plus Protein™ Dual Color standards are a mixture of ten recombinant proteins (10–250 kD)， including eight blue-stained bands and two pink reference bands (25 nd 75 kD).
