ERK 2 Background Information
Mitogen-activated protein kinase （MAPK） signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 （ERK 1, p44） and 2 （ERK 2, p42）. Growth factors, steroid hormones, G protein-coupled receptor ligands, and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK1 and ERK2 requires phosphorylation by upstream kinases such as MAP kinase kinase （MEK）, MEK kinase and Raf-1. ERK1 and ERK2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the Threonine-Glutamate-Tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK2 gene maps to chromosome 22q11.21 and encodes a 360-amino acid protein.

• rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-154-G
• epitope mapping at the C-terminus of ERK 2 of rat origin
• recommended for detection of ERK 2 p42 and, to a lesser extent, ERK 1 p44 of mouse, rat, human, avian, frog, zebrafish and Xenopus origin by WB, IP, IF, IHC（P）, FCM and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
• blocking peptide, sc-154 P
• agarose conjugate for IP studies, sc-154 AC, 500 µg/0.25 ml agarose
• fluorescein （sc-154 FITC） and rhodamine （sc-154 TRITC） conjugates for immunofluorescence, 200 µg/ml
• phycoerythrin （sc-154 PE） conjugate for intracellular FCM, 100 tests
• Alexa Fluor® 405 （sc-154 AF405）, Alexa Fluor® 488 （sc-154 AF488） and Alexa Fluor® 647 （sc-154 AF647） conjugates for FCM or immunofluorescence; 100 µg/2 ml

上海力敏實(shí)業(yè)有限公司代理santacruz抗體產(chǎn)品，咨詢(xún) 
上海力敏實(shí)業(yè)有限公司代理santacruz抗體產(chǎn)品，咨詢(xún)