Overview
Product name
Anti-RANK antibody [64C1385]
See all RANK products （9） ...
Description
Mouse monoclonal [64C1385] to RANK
Tested applications
IHC-P, IP, WB, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant fragment containing the extracellular domain of RANK （Human）.
Positive control
Activated T cells or RAW （a mouse macrophage monocyte）.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term （1-2 weeks）. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
- 100 µg at 0.5mg/ml
Purity
Protein G purified
Clonality
Monoclonal
Clone number
64C1385
Isotype
IgG1
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Growth Factors/Hormones >> TNF
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
- Datasheet PDF
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Immunoprecipitation - RANK antibody [64C1385] （ab13918）
![Immunoprecipitation - RANK antibody [64C1385] （ab13918） image](https://img74.foodjx.com/bb33f2c9b6dc3b85eb515dd667773c4a436e3e2892431c0e9379ecf37d077222163174358feef235.png)
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Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Anti-RANK antibody [64C1385] （ab13918）
![Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - [64C1385] （ab13918） image](https://img75.foodjx.com/bb33f2c9b6dc3b85eb515dd667773c4ac2d06b6608c11aa145f191f1b4905dbcb8c2fbbb3e910cf6.jpg)
（enlarge）
Applications
Our Abpromise guarantee covers the use of ab13918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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IP: Use a concentration of 1 - 2 µg/ml.
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WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximay 97 kDa.
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Flow Cyt: Use 5µg for 106 cells.
Target
Function
Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.
Tissue specificity
Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
Involvement in disease
Defects in TNFRSF11A are the cause of familial expansile osteolysis （FEO） [MIM:174810]. FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. The osteolytic lesions develop usually in the long bones during early adulthood. FEO is often associated with early onset deafness and loss of dentition.
Defects in TNFRSF11A are a cause of Paget disease of bone type 2 （PDB2） [MIM:602080]; also known as familial Paget disease of bone. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.
Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 （OPTB7） [MIM:612301]; also called osteoclast-poor osteopetrosis with hypogammaglobulinemia. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia.
Sequence similarities
Contains 4 TNFR-Cys repeats.
Cellular localization
Membrane.
Alternative names
- Activator of NFKB antibody
- CD 265 antibody
- CD265 antibody
Database links
- Entrez Gene: 8792 Human
- Entrez Gene: 21934 Mouse
- Omim: 603499 Human
- SwissProt: Q9Y6Q6 Human
- SwissProt: O35305 Mouse
- Unigene: 204044 Human
- Unigene: 6251 Mouse
[64C1385] images:
Immunoprecipitation - RANK antibody [64C1385] （ab13918）
Detection of RANK in RAW cells. Lane 1: mouse cell line. Lane 2 & 3: IP/Western blot analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody （lane 2） or ab13918 （lane 3）, and detected with ab13918.
Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - [64C1385] （ab13918）
IHC image of ab13918 staining in human t cell lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer （pH6, epitope retrieval solution 1） for 20 mins. The section was then incubated with ab13918, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems （automated and non-automated） customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
