人類基因組DNA(5 µg)用(左到 右)Bg/I, HindIII,或NheI 酶切,在 1% Certified 分子生物瓊脂糖凝膠 上分離,5"Hg 真空轉(zhuǎn)印90 分鐘到 Zeta-Probe GT 膜。印跡膜用32P 標(biāo)記的VIII 因子片段雜交,X 光片 曝光過夜。探針由Genetic Institute of Boston 提供。
左到右,1.0、2.5、5、10或20µg CHO HA-1細(xì)胞總RNA乙醛凝膠分離,3"真空轉(zhuǎn)印90分鐘到Zeta-Probe GT 膜。印跡膜用,32P標(biāo)記的hsp70 cDNA 片段雜交,X光片曝光過夜。探針和 RNA由Gloria Lee, UCSF, USA提供。
目錄 # | 描述 |
Vacuum Blotter | |
165-5000 | Model 785 Vacuum Blotter With Regulator, includes vacuum regulator, base with vacuum stage, porous vacuum plate, reservoir seal O-ring, sealing frame, assorted window gaskets, lid, instructions |
165-5001 | Model 785 Vacuum Blotter System, 100/120 V, includes vacuum pump, vacuum regulator, base with vacuum stage, porous vacuum plate, reservoir seal O-ring, sealing frame, assorted window gaskets, lid, instructions |
165-5002 | Model 785 Vacuum Blotter System, 220/240 V |
165-5003 | Model 785 Vacuum Blotter Basic Unit, includes base with vacuum stage, porous vacuum plate, reservoir seal O-ring, sealing frame, assorted window gaskets, lid, instructions |
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